15th July

Sriram

Cool. I got colonies in all the plates. Still more to do...

Weenink

The BBa_K145280 had one colony. other ones didnt. It is not reccomendable to use this super fast protocol as it is very unreliable. 30 ml of LB 1x amp. was inoculated with this colony and put in a shaking incubator at 37 degrees and 160 rpm at 10:15.