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Overview Sensitivity Tuner --- Characterisation --- Modelling Colour Generators --- Carotenoids (Orange/Red) --- Melanin (Brown) --- Violacein (Purple/Green) The Future Safety

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Overview

Previous iGEM teams have focused on genetically engineering bacteria to respond to novel inputs – for example light, or biologically significant compounds. There is an unmistakable need, therefore, to also develop clear, user-friendly outputs, especially for use with biosensors. The most popular output is the expression of a fluorescent protein, detectable using fluorescence microscopy. However, how much easier would it be if we could simply see the output with our own eyes?

The Cambridge 2009 iGEM team is engineering E. coli to produce different pigments in response to different concentrations of an inducer. Our device is a three part system:

Sensor: Our sensor system is sensitive to different concentrations of an inducer.

Adaptor: The adaptor is an amplifier; its role in the system is to ensure large-scale production of the genes necessary for pigment production no matter the Pops input from the sensor. This amplification acts as an "on" switch to guarantee maximum pigment production even with low level input. The amplifier is also responsible for the sensitivity to the inducer.

Colour: Pigment production

Thus, we can develop a population of mixed strains of bacteria. The concentration of the inducer to which they are exposed determines which strains are activated to produce pigment.