Template:Team:KULeuven/5 August 2009/VanillinProduction

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Miniprep of pairs:

  • RBS + Sam5 conc: 101,3 ng/ul
  • RBS+Sam8 conc: 95.7 ng/ul
  • Sam8 conc: 81,8 ng/ul
  • ComT , conc: 122,9 ng/ul


Restriction digestion

  • Sam8, EcoRI en SpeI
  • Sam5, EcoRI en XbaI


  • RBS, EcoRI en SpeI
  • ComT, EcoRI en XbaI


  • Fcs, EcoRI en SpeI
  • Ech, EcoRI en XbaI

The mixture was incubated in 37C for 1h30

Followed by gelelectroforesis of the digested parts

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