Team:Newcastle/Labwork/7 August 2009

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Lab Session 07/08/09

Stochastic Switch Team

Today, we identified the strains and prepared the spectinamycin solution.

We will use the E.coli strain numbered as 2386.

B. subtilis 168 strain is already in the fridge. To test our biopbricks ww will use gfp-rrnb integration vector. It uses spectinamycin as the antibiotic,

We solved 65mg of the spectinamycin in 13 ml of water, purified the solution and placed it into the fridge. 

Prepared volume of the spectinamycin solution: 13 ml
Concentration: 5mg/ml


Sporulation Tuning/ Chassis Team

Today, we prepared Lysozyme stock solution in preparation for the recovery of our cwlD spores kindly sent to us by Anne Moir from Sheffield University.

Referring to Method A of the protocol, it states that there should be 200ug per ml of buffer solution present.

We need: 

200ug Lysozyme in 100ul DI Water
2mg Lysozyme in 1ml DI Water

We made 0.5g of Lysozyme in 10ml of DI Water, where: 

0.5g Lysozyme in 10ml DI Water
50mg Lysozyme in 1ml DI Water
50ug Lysozyme in 1ul DI Water




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