Team:Newcastle/Labwork/18 August 2009

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Contents

Lab 18/08/09

Sporulation Tuning/ Chassis Team

Summary

[[Image:Newcastle 18 August BSub fume.jpg|thumb|The single colonies in the fume cabinet.] [[Image:Newcastle 18 August BSub grown single.jpg|thumb|The single colonies.] [[Image:Newcastle 18 August BSub in fume 1.jpg|thumb|The single colonies in the fume cabinet.] [[Image:Newcastle 18 August BSub in fume 2.jpg|thumb|The single colonies in the fume cabinet.] [[Image:Newcastle 18 August BSub in fume.jpg|thumb|The single colonies in the fume cabinet.]

cwlD plates 10-1 and 10-2.
cwlD plates 10-3 and 10-4.
cwlD plate 10-5.
cwlD plates 1 and control.
Jane plating out sleB and cwlJ germinated spores.

Today, the moment of truth, the recovery of spores cwlD seemed to have worked judging from the results. A big sigh of relief from our team. Since it has been confirmed that the problem was that insufficient lysozyme stock solution was added, and that the current amount of lysozyme stock solution now works, we proceeded with the treatment for the recovery of spores sleB and cwlJ. The only and main difference is that the spores after treatment were plated out on plates with Kan and Em antibiotic.

We also did the iodine test which came back with relatively good results.

Lastly, we made up a new batch of LB solution for common use.

Results

Recovery of spores sleB and cwlJ



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