Team:Newcastle/Project/Labwork/OurProtocols/Aspirator
From 2009.igem.org
Using the Aspirator
- Make sure the valve of the aspirator is set to the closed position; if in the closed position, switch on the pump.
- Switch on the cooler.
- Set 'Drying Rate' to "HIGH"
It is important that the pump and the cooler have been running for at least 10 minutes prior to carrying out aspirations - this gives the machine time to reach the correct temperature.
- Open the aspirator lid and insert the Eppendorf tubes making sure that the lids are open. Also ensure that the Eppendorf tubes are placed in a symmetrical fashion so that a balance is maintained.
- Set the 'Concentrator' to "ON"
- Slowly set the valve to the open position.
- Leave samples in the aspirator for 5 minutes initially, checking on it's progress afterwards.
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