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Week 7

Monday

Wet Work

Amplification

Colony PCR of 8 colonies picked from 391 in pSB3K3 transformants and 8 colonies picked from 374 in pSB3K3 transformants, overnight cultures of each of those colonies as well.

MelA BioBrick

PCR done of the origonal MelA and the MelA inbtermediate to biobrick (without the second PstI site). This was too create enough DNA for a resitriction digest to be carried out to check that the previous weeks PCRs had been sucessful. After PCR, the products were run on a gel and extracted.

We also tested the Primer C again, as it didn't work last time. Three PCR's were done using the same Primer C, a different aliquot of Primer C and re-vortexed Primer C from the origonal vial. None of these worked, so the primer C i have is not working.

Dry Work