Keep track with what we did so far

(15.08.09)

This sixth week of wetlab we have done the following things:

• Protocol of ligation was refined.
• !!! Inducible LOVTAP and Read out biobrick were created.!!! The system is completed.
• They need to be sequenced, characterized and submitted to the registery.

(08.08.09)

This fifth week of wetlab we have done the following things:

• Problem in ligations.

(01.08.09)

This fourth week of wetlab we have done the following things:

• Our gene of interest (photoreceptor LOVTAP) was cloned in front of a terminator (iGEM part [http://partsregistry.org/Part:BBa_B0015 BBa_B0015]). We created our first biobrick.
• The second biobrick was coloned as well, but we wait for the results to confirm that the plasmid contain the right insert
• Modeling part: Preliminar simulations were lauched, and their analysis were done. Simulations will be lauched in few days.

(24.07.09)

This third week of wetlab we have done the following things:

• Again : Several attempts to create the first biobrick (Our gene of interest (photoreceptor LOVTAP) in a iGEM plasmid containing a terminator) and the second biobrick (A lacI promoter in front of an RBS), but no results were obtained for the first biobrick neither for the second one.
• Modeling part: Preliminar simulation was launched over the weekend.

(17.07.09)

This second week of wetlab we have done the following things:

• Several attempts to create the first biobrick (Our gene of interest (photoreceptor LOVTAP) in a iGEM plasmid containing a terminator) and the second biobrick (A lacI promoter in front of an RBS), but no results were obtained for the first biobrick neither for the second one.
• Modeling part: Files required to launch simulations were created and analyzed.

(12.07.09)

This first week of wetlab we have done the following things:

• Transformed the plasmids with the LovTAP gene, generously sent by Dr. Sosnick's lab from Chicago universtiy, into competent E.Coli
• Designed the cloning strategy for cloning the LovTAP gene from its original vector to a iGEM vector+add an inducible promoter (LacI) (+RBS +term.)
• Ordered and received the primers needed for the PCR of LovTAP
• Designed the cloning strategy for inclusion of the LovTAP BioBrick with different reporting cassettes
• Transformed all the BioBricks that will be needed for the cloning strategies (c.f. notebook for more information about these parts) into competent E.Coli
• Fused the two BioBricks "LacI" and "RBS"
• Digested the LovTAP PCR products and RBS part