Team:Imperial College London/Wetlab/Protocols/PromoterCharacterisation

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Calibration protocols (CA)

Section Assay Overview and Aims
2.Auto-Induction IPTG
  • Characterise Lac promoter by varying concentrations of IPTG
  • Determine the IPTG concentration that allows for maximal protein production while still being non-toxic to the cell

Mr. Gene   Geneart   Clontech   Giant Microbes

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  • Characterise Lac promoter by varying concentrations of IPTG
  • Determine the IPTG concentration that allows for maximal protein production while still being non-toxic to the cell

|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"

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