Results Recombinase

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Recombinase - Results

And those pretty pictures were created, following this protocol.

PCR product of pR6K-KanR.JPG PRhaFlp-CmR digested with EcoRI .JPG Re-electroporation of pRhaFlp-CmR, digestedwith EcoRI .JPG PRhaFlp-CmR-KanR, digested with PvuII.JPG Re-electroporated pRhaFlp-CmR-KanR ,digested with PvuII.JPG PTetFlp-KanR, digested with Pst I.JPG Re-electroporation of pTetFlp-KanR with Pst I and EcoRI digest.JPG F3-ZeoR-F3-RFP digest with PvuII.JPG PTetFlp-KanR+F3-ZeoR-F3-RFP, digested with PstI.JPG

Re-electroporated pTetFlp-KanR+F3-ZeoR-F3-RFP, digested with PstI.JPG
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