Team:BIOTEC Dresden/Methods Vesicles

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Vesicles - Methods

Setup of the microfluidic system

The microfluidic system consists of a flow chamber created out of Polymethylsiloxane (PDMS)- an organic silicon based polymer, and a pump system that controls the rates of flow of the various liquids into the chamber. Droplets are created within a defined space in the chamber and are propagated along a grid that allows temporary containment and imaging.

Production of flow chambers:

  • mix PDMS and curing agent in 10:1 ratio
  • degas and pour on wafer with etched microstructures
  • polymerize on heat plate at 150 ºC for 30 min
  • add unpolymerized PDMS mixture to points on microstructure where microtube inlets are to be pierced
  • polymerize on heat plate at 150 ºC for 30 minutes
  • remove polymerized PDMS from wafer, cut to fit onto glass cover slide (24 x 60 mm), and use clean needles (0.8 mm) or laser cutter to pierce tube inlets
  • ionize PDMS and glass slide in plasma chamber for 30 sec to make it reactive
  • align PDMS on glass slide and seal
  • seal irreversibly by heating on plate at 60ºC for 6 hours







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