Lab Aug 5 2009

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Miniprep 4 previous parts (B0015 Amp/Kan, J06504 Amp, R0051 Amp, B0034 Amp) from overnight cultures: Qiagen Miniprep Protocol

gel 4 parts: Agarose Gel Electrophoresis Protocol

visualized the gel, pasted into Ky's notebook

nanodropped the RFP generator following the miniprep, and using TE as the elution buffer to zero the nanodrop

21 ng/ul

260/280 ratio of 1.54

260 to 230 ratio of 1.81

R0051 - lambda cI promoter, we re-ran the transformation protocol with more of the rehydrated pklasmid

Rehydrated and Transformed the following parts:

I0050 - arabinose promoter on a Kan plate, add IPTG to broth culture to induce high copy

R0010 - lactose promoter

J23032 - ribolock RBS

J23066 - ribokey

C0051 - lambda cI with the LVA tag