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Team:Groningen/Project
From 2009.igem.org
[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]
| The Project
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Planning and requirements:
- Gasvesicle:
- Modelling:
- Buoyancy
- Permeability
- number of gasvesicles
- Where do the gasvesciles end, in hight.
- Mass of the bacteria (e.coli)
- How long it takes before it floates
- how long it take untill it is being expressed
- How long it will take untill there are enough gasvesicles
- how does it stay floating
- Buoyancy
- Lab
- GvP
- Cluster of biobricks
- Vector ordered from article
- first expression with constutatieve promotor, later with metal sensitive promotor
- Measurements
- Where are the bacteria, what are bac concentrations on a certain hight
- Making pictures on certain hight and compare with picture of known concentration
- Where are the bacteria, what are bac concentrations on a certain hight
- GvP
- Modelling:
- Metal importers
- Modelling
- Import speed
- Amount
- Max
- Lab
- HmtA
- Zn/Cu alone
- B-type ATPase (could be use if there is a ATP shortage?)
- CitM
- Divalent ions
- Citrate around
- Citrate can bind metals that are already bound.
- Measurements
- Transporter, on/off mechanism, up to what concentration (in the cell) does it still have metal uptake.
- Measure concentration of metal. difference between begin and end concentrations of metal outside the cell
- HmtA
- Modelling
- Promotors
- Modelling
- How do they react to metal, metal concentration vs expression of the protein after promotor (bv GFP)
- Lab
- Measuments
- Expression of the gene behind the promotor(GFP)
- Cu-promotor
- Arc promotor
- Measuments
- Modelling
- Accumulation
- Modelling
- Speed
- Metaliotheines concentration
- Lab
- Measurements
- Measure accumulation. By measuring before/after concentration metal with and without accumulation protein.
- Biobrick Bba_K129004
- Rest
- Measurements
- Modelling
Order of action
- Buoyancy
- Metal importation
- Accumulation
- Metal sensitive promotor
Retrieved from "http://2009.igem.org/Team:Groningen/Project"
