Team:Todai-Tokyo/Protocols/Notebook Sample

From 2009.igem.org

Revision as of 02:44, 20 October 2009 by Craig (Talk | contribs)
UT.png
Home The Team The Project Parts Submitted to the Registry Modeling Notebook Protocols Ethics



Contents

Miniprep

The following were miniprepped:

  • pLacI-RFP-dterm (From Ligation on 9/4; 4 colonies)
  • RFP ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 BBa_J04450] from [http://partsregistry.org/assembly/libraries.cgi?id=19 2009 Spring Distribution]; 6 colonies)

PCR

The yqiT gene was PCR amplified from pLacI-yqiT-dterm (miniprepped on 9/7) using the following primers:

yqiT fwd: agcccgtgtagtactgtagagtt
yqiT rev: agggatgtttgccagtcgtaattag

using PCR program 1 and Ex-taq.

This PCR reaction attaches a Biobrick prefix and suffix to the gene.


Sequencing


Transformation

Ligation

Infusion

Gel Purification

RE Digest

Colony PCR

Retrieved from "http://2009.igem.org/Team:Todai-Tokyo/Protocols/Notebook_Sample"