Team:Brown/Notebook Recipes/SOB

From 2009.igem.org

Revision as of 06:53, 20 October 2009 by Ivorugan (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Making SOB Medium ( For growing competent E.Coli cells)

• Making 1L of SOB • Please look at Pg 1356 of the Lab Reference Book for details. 1. 20g Bacto tryptone 2. 5 g of Bacto yeast Extract 3. 0.584g NaCl 4. 0.186g KCl 5. 1L ddH2O 6. 2.4g MgSO4 (OR MgCl2 in 90ml ddH2O ) ➢ Swirl the flask until the ingredients are completely dissolved. ➢ Autoclave the medium.

Retrieved from "http://2009.igem.org/Team:Brown/Notebook_Recipes/SOB"