Team:Brown/Notebook Recipes/SOB

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Making SOB Medium ( For growing competent E.Coli cells)

• Making 1L of SOB • Please look at Pg 1356 of the Lab Reference Book for details. 1. 20g Bacto tryptone 2. 5 g of Bacto yeast Extract 3. 0.584g NaCl 4. 0.186g KCl 5. 1L ddH2O 6. 2.4g MgSO4 (OR MgCl2 in 90ml ddH2O ) ➢ Swirl the flask until the ingredients are completely dissolved. ➢ Autoclave the medium.

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