Team:Todai-Tokyo/Protocols/PCR/programs

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PCR Programs

Pfu Standard

  1. 95ºC 2 min
  2. 95ºC 30 sec
  3. 52-55ºC 30 sec （Depending on the Tm of the primers）
  4. 72.5ºC 15 sec × （# of kb of DNA to be amplified）
  5. 95ºC 30 sec Repeat 2-4 29 times
  6. 25ºC pause
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