Team:KU Seoul/Result

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Results

1. Synthetic Circuit - I : pSB3C5/Pars-gfp-Pznt-rfp
1)Pznt-rfp
Zn2+ (mM) 0 0.5 0.75 1 1.5 2
OD600 0.490 ± 0.003 0.459 ± 0.004 0.433 ± 0.008 0.418 ± 0.005 0.275 ± 0.011 0.243 ± 0.008
Red fluorescence (RF) 26628 ± 706 27856 ± 292 29333 ± 292 31217 ± 66 46044 ± 237 49188 ± 174
OD600/RF 54376 ± 1203 60646 ± 787 67803 ± 573 74687 ± 755 167833 ± 7202 202565 ± 6428
Background 0 6270 ± 520 13427 ± 1719 20311 ± 475 113457 ± 6016 148189 ± 6731

KU Seoul Result RFP.png

2)Pars-gfp
AsO3- (mM) 0 0.00625 0.125 0.25 0.5 0.75 1
OD600 0.553 ± 0.009 0.519 ± 0.002 0.480 ± 0.005 0.395 ± 0.007 0.315 ± 0.006 0.286 ± 0.004 0.273 ± 0.006
Green fluorescence (GF) 27740 ± 226 26411 ± 169 25267 ± 267 25536 ± 213 25407 ± 259 24849 ± 152 26479 ± 149
OD600/GF 50206 ± 1225 50855 ± 123 52606 ± 689 64669 ± 1684 80665 ± 603 86897 ± 1321 96784 ± 2165
Background 0 648 ± 1314 2399 ± 969 14463 ± 2896 30459 ± 1631 36691 ± 1636 46578 ± 3220

KU Seoul Result GFP.jpeg KU Seoul Calibrationcurve.jpeg

2.Synthetic circuit – II : PyodA-AMD
Cd2+ (mM) 0 0.05 0.1 0.2 0.4 0.8
OD615 0.242 ± 0.005 0.258 ± 0.008 0.274 ± 0.011 0.284 ± 0.01 0.293 ± 0.011 0.302 ± 0.01
Background 0 0.016 ± 0.004 0.032 ± 0.006 0.043 ± 0.005 0.052 ± 0.007 0.060 ± 0.007

KU Seoul Expression.jpeg

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