Team:Groningen/Parts/Submitted Parts

From 2009.igem.org

Submitted Parts

TODOMaybe some general information about our submitted parts.

• To get to the partsregistry site of a particular part one can click on the name of the part
• To see what our main findings where (no details or derivations) regarding a particular part one can click on more information

Importers

GlpF
About 100 to 200 words chracterizing the Parts ... bla...

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_K190028:Design More Information].

GlpF (with strong RBS)
About 100 to 200 words chracterizing the Parts ... bla...

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_K190061:Design More Information].

GlpF regulated by medium constitutive promotor
About 100 to 200 words chracterizing the Parts ... bla...

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_K190062:Design More Information].

GlpF regulated by pLacI
About 100 to 200 words chracterizing the Parts ... bla...

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_K190063:Design More Information].

HmtA (contains PstI sites)
About 100 to 200 words chracterizing the Parts ... bla...

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_K190018:Design More Information].

Acummulators

fMT
About 100 to 200 words chracterizing the Parts ... bla...

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_K190019:Design More Information].

fMT regulated by low constitutive promotor
About 100 to 200 words chracterizing the Parts ... bla...

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_K190031:Design More Information].

fMT regulated by pLacI
About 100 to 200 words chracterizing the Parts ... bla...

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_K190032:Design More Information].

MBP-ArsR (fusion protein)
About 100 to 200 words chracterizing the Parts ... bla...

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_K190027:Design More Information].

( MBP-ArsR (with strong RBS)
About 100 to 200 words chracterizing the Parts ... bla...

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_K190071:Design More Information].

( MBP-ArsR regulated by pLacI
About 100 to 200 words chracterizing the Parts ... bla...

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_K190072:Design More Information].

Promotors

Arsenic Promoter
Promoter sequence with recognition site for ArsR transcriptional regulator protein. ArsR binds to the promoter sequence in the absence of As and releases on binding of As, thereby activating transcription.

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_K190015:Design More Information].

Arsenic Promoter (with wild type RBS)
Promoter sequence containing the recognition site for ArsR transcriptional regulator protein. Downstream from the promoter is the RBS that is attached to this promoter region in the E. coli genome. ArsR binds to the promoter sequence in the absence of As and releases on binding of As, thereby activating transcription.

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_K190023:Design More Information].

Zinc Promoter
Promoter sequence with recognition site for ZntR transcriptional regulator protein. ZntR activates transcription when Zn(II) is bound.

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_K190016:Design More Information].

Zinc Promoter (with wild type RBS)
The pZntR from E.coli K.12 has a specific RBS site behind it in the genome. Here the RBS site is attachted to the promoter region. The RBS site might influence the activity of the promoter and will be tested in the same way as BBa_K190016. ZntR activates transcription when Zn(II) is bound.

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_K190022:Design More Information].

Copper Promoter
Promoter sequence with recognition site for the CueR transcription regulating protein. When copper levels in the cell rise CueR activates transcription.

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_K190017:Design More Information].

Copper Promoter (with wild type RBS)
Promoter sequence with recognition site for the CueR transcription regulating protein and its downstream RBS (as it is found in the E. coli genome). When copper levels in the cell rise CueR activates transcription.

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_K190024:Design More Information].

GVP Constructs

( GVP Gas Vesicle Proteins
GVP consists of 6064 base pairs. It's Backbone is With a resistance against Ampicillin/Gentamycin

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_I750016:Design More Information].

GVP regulated by low constitutive promotor
The Gas Vesicle gene cluster (BBa_I750016) submitted by Melbourne 2007 team and the constitutive promoter BBa_J23109 were combined, and the product ligated into vector pSB1AC3. The construct was used to test and caracterize the gas vesicle formation.

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_K190025:Design More Information].

GVP regulated by medium constitutive promotor
The Gas Vesicle gene cluster (BBa_I750016) submitted by Melbourne 2007 team and the constitutive promoter BBa_J23106 were combined, and the product ligated into vector pSB1AC3. The construct was used to test and caracterize the gas vesicle formation.

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_K190026:Design More Information].

GVP regulated by pArsR
The Gas Vesicle gene cluster (BBa_I750016) submitted by Melbourne 2007 team and the metal sensitive promoter BBa_K190015 were combined, and the product ligated into vector pSB1AC3. The construct was used to test and caracterize the gas vesicle formation.

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_K190033:Design More Information].

GVP regulated by pZntR
The Gas Vesicle gene cluster (BBa_I750016) submitted by Melbourne 2007 team and the metal sensitive promoter BBa_K190016 were combined, and the product ligated into vector pSB1AC3. The construct was used to test and caracterize the gas vesicle formation.

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_K190034:Design More Information].

GVP regulated by pCueO
The Gas Vesicle gene cluster (BBa_I750016) submitted by Melbourne 2007 team and the metal sensitive promoter BBa_K190017 were combined, and the product ligated into vector pSB1AC3. The construct was used to test and caracterize the gas vesicle formation.

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_K190035:Design More Information].

GVP regulated by pLacI
About 100 tThe Gas Vesicle gene cluster (BBa_I750016) submitted by Melbourne 2007 team and the LacI and CAP regulated promoter BBa_R0010 were combined, and the product ligated into vector pSB1AC3. The construct was used to test and caracterize the gas vesicle formation.

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_K190036:Design More Information].

Devices

Arsenic Accumulation Device (IPTG induced)
About 100 to 200 words chracterizing the Parts ... bla...

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_K190038:Design More Information].

Arsenic Accumulation Device (constitutive)
About 100 to 200 words chracterizing the Parts ... bla...

Have a look at the part design page on the registry for [http://partsregistry.org/Part:BBa_K190039:Design More Information].

(( Arsenic Accumulation Device (IPTG induced, able to regulate pArsR)
About 100 to 200 words chracterizing the Parts ... bla...