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Uppsala-Sweden/30 September 2009
From 2009.igem.org
Horrible Transformation results
All the transformations forma yesterday failed, the purified digestions were run on a gel to figure out if the problem were related to us loosing all the DNA in the purification step.
The result turned out to be as we suspected, basically all the DNA was gone.
Evaluation of Nucleospin PCR Clean-Up kit
DUe to the results we decided to evaluate the Nuclespin kit, we used different columns, amount of water, wash buffer and dilutions of NT buffer.
The result was that there is no difference in we use our own wash buffer or other binding columns. But if the NT buffer is diluted the recovery is way much lower than what is said in the manual for DNA fragments of our size.
