Team:Imperial College London/Wetlab/Results/OD calibration

From 2009.igem.org

Revision as of 22:47, 20 October 2009 by Kun (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)



We have calibrated the absorbance at 600nm (O.D.600) with the colony forming units. This allows us to approximate our absorbance data into a cell count number. We can now normalise fluorescence data to obtain the fluorescence produced per cell.

We performed the experiment and further reruns with 2-3 repeats per OD value and for 2 different dilutions.



Mr. Gene   Geneart   Clontech   Giant Microbes