Team:Groningen/Future

From 2009.igem.org

Ideas for future work (TODO references):

• Reusing the bacteria by moving them to another container and using Sb to accelerate As efflux (Meng2004).
• Demonstrating the presence of arsenic by putting Luciferase under regulation of ArsR.
• Use hydrophobic chaplin proteins to keep the bacteria floating on the surface of the medium (it won't help them to start floating)
  • This might be used to start bouyancy with GVP and keep them on the surface of the medium with these proteins. These proteins origin from Gram positive bacteria, which may cause problems. Information may be asked from: Dennis Klaassen (MolMic).
• Safety
• Summer events like the Kluyver day?
• Using the ability of the roots of some plants (unknown which exactly have this feature) to solubilize metal ions from solid metal in combination with our bacterium could lead to improved detoxification of the environment as also peaces of copper or zinc waste etc.

Labwork

• Removing repeat of GVP
• Completing SmtA
• Completing HmtA
• Characterize the uptake capacity of transporters
• Nitrogen sparging in fermentation to see the effect of oxidation states of heavy metals
• Use higher concentrations in death assays

Modeling