Notebook/June.html

June 29th, 2009

We isolated vectors containing d53 and Dimtetra. Gel electrophoresis showed that isolation was successful. BL21DE3 pLysS cells (for protein production) were transformed with the isolated vectors.
Other group of BL21DE3 pLysS cells was inoculated on LB plates with chloramphenicol.

We multiplied ccdB domain and p53 monomer with PCR using ccdB-f, ccdB-r, p53-f and p53-r primers.

June 30th, 2009

Gel electrophoresis of PCR products (see June 29th).
BL21DE3 pLysS cells transformed with vectors, containing d53 and Dimtetra, were inoculated into 10 mL of LB+antibiotic.