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Team:Newcastle/Modelling/SporulationTuning
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Sporulation Tuning
KinA Expression Model
Under normal conditions, LacI represses KinA.
However, in the presence of IPTG, KinA can be expressed, as IPTG binds to LacI, deactivating it. Equations (a) and (b) describes how IPTG binds to LacI, forming LacI*, which is the deactivated form of LacI
Over time, the deactivated form of LacI, LacI* may degrade. This is also taken into consideration while building the model.
Protein synthesis requires two steps, transcription and translation, and is further illustrated in Figure 1, below.
Referring to Figure 1, the following equations can be written:
- Transcription of LacI,
- Translation of LacI,
where mRNA_LacI is inducing the formation of LacI.
- Transcription of KinA,
where LacI is repressing mRNA_KinA, therefore a lower concentration of LacI would result in a higher concentration of mRNA_KinA.
- Translation of KinA,
where mRNA_KinA is inducing the formation of KinA.
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
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