From 2009.igem.org

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Project introduction. Inspired by the natural regulator of circadian bioclock exhibited in most eukaryotic organisms, our team has designed an E.coli-based genetic network with the toxin-antitoxin system so that the bacterium oscillates between two states of dormancy and activity (more...)

Parts & devices

Parts overview

Go to [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=SJTU-BioX-Shanghai PartsRegistry] to see our submited parts.

DNA gel electrophoresis verification

Almost all of our parts have been detected after enzyme digestion. The following DNA electrophoresis pictures prove our parts to be accurate and reliable.

Lane2: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185000 BBa_K185000] RelE toxin+Rbs30 311bp OK
Lane3: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185001 BBa_K185001] Arabinose-induced RelE Generator 1791bp Failed
Lane4: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185002 BBa_K185002] Lon Protease+Double terminater 2492bp OK
Lane5: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185003 BBa_K185003] RelE toxin+Lon proteas 2827bp OK
Lane6: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185004 BBa_K185004] RelE toxin+Double terminator 443bp OK

Lane3: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185028 BBa_K185028] RelB+Double-terminator 395bp OK

Lane3: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185007 BBa_K185007] Promoter116+RBS34+lLacI+Double-terminator+Plac+RBS31 1514bp OK
Lane5: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185016 BBa_K185016] Promoter118+RBS34+LacI+Double-terminator+Plac+RBS31 1514bp OK
Lane15: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185018 BBa_K185018] Promoter118+QPI+RBS30+RelB+Double-terminator 1454bp OK
Lane17: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185036 BBa_K185036] Promoter110+QPI+RBS34+RelB+Double-terminator 1433bp OK
Lane20: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185011 BBa_K185011] Promoter116+QPI+RBS31+RelB+Double-terminator 1435bp OK

Lane13: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185053 BBa_K185053] Promoter116+RBS34+LacI+Double-terminator+pPlac+RBS31+RelE+RBS30+Lon protease+Double terminator 4347bp OK

Lane10: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185012 BBa_K185012] Promoter116+RBS34+LacI+Double-terminator+Plac+RBS34 1347bp OK
Lane12: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185019 BBa_K185019] Promoter118+RBS34+LacI+Double-terminator+Plac+RBS34 1512bp OK

Lane 2&3: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185045 BBa_K185045] Composite J23110+Q01121 1416bp OK
Lane 4&5: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185046 BBa_K185046] Composite J23110+Q01121 1416bp OK
Lane 6&7: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185042 BBa_K185042] Composite J23110+Q04510 1030bp OK
Lane 8&9: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185043 BBa_K185043] Composite J23116+Q04510 1030bp OK
Lane 10&11 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185044 BBa_K185044] Composite J23118+Q04510 1030bp OK

Lane 5&6&7: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185037 BBa_K185037] Composite J23116+Q04510+B0031 1052bp OK
Lane 8-12: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185038 BBa_K185038] Composite J23116+Q04510+B0034 1050bp OK
Lane 15&17&18&19: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185039 BBa_K185039] J23118+Q04510+B0030 1053bp OK
Lane 20&21: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185040 BBa_K185040] J23118+Q04510+B0031 1052bp OK

Lane 2-8: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185034 BBa_K185034] Device J23116+Q04510+B0034+K185048 1433bp OK

Lane 4: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185005 BBa_K185005] Generator J23116+Q04510+B0034+K185048+K185005 3298bp OK

Lane 2-9: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185051 BBa_K185051] Composite J23110+B0034+BBa_K185008+BBa_K185009 2748bp PCR analysis (plasmid backbone added) OK

Lane 2, 4, 6, 7: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K185052 BBa_K185052] Regulatory 1342bp PCR analysis (backbone sequence added) OK