Uppsala-Sweden/12 October 2009

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Evaluating in e.coli

The colony PCR from yesterday were run on a gel for evaluation and the result was that we had inserts of the correct length for ePRPRAT and ePRPRYT but not for ePRPRAT.

Various

BG-11 plates with kanamycin and without CuSO4 were also made for triparental mating.

--Anders 17:09, 11 October 2009 (UTC)

Transformation

Digestion of PRKRYT 2 on AC (move to carrier plasmid), PRPRAT on AC (move to carrier plasmid) and PRPRYT AK (move to AC)