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Team:Newcastle/ Stochastic Switch cloning strategy
From 2009.igem.org
Project
Sub-projects
Wet Lab
Meetings
Planning
Links
Stochastic Switch Cloning Strategy
We have the following cloning strategies for testing our construct.
Lab Work Strategies
Cloning strategies
Currently in the lab we are half way to acheiving a complete manual brick which is the degradation controller. In the lab so far, the stochastic switch team have:
- Rehydrated, transformed, and miniprepped the biiobricks involved in promoter replacement, as well as frozen down these E.coli strains into the TPA collection.
- Prepared the pSB1AT3 plasmid backbone (restriction digest, gel extraction) ready for ligation.
- Have successful PCR products for the sac region (bacillus) and sspb (E.coli), and a possible success for the ara region.
- Have done digests for sspb and sac, sspb has been extracted from the gel and is due for cloning.
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]
