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Receptors

Removal

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Contents 1 Experimental Results 2 Sequences, Primers 3 PCR results 4 Parts 4.1 K195100-K195199 4.2 K195200-K195299 4.3 K195300-K195399 4.4 K195400-K195499 4.5 K195600-K195699

Experimental Results

Protocols

Sequences, Primers

Sequences, primers and PCR results by subteam:

• Virus Proteins

PCR results

• Virus Proteins
• Removal Lab Note

Parts

K195100-K195199

Cellular Receptors: CD4

Part Name	Picture	Part Type	Part Length	Subparts	Short Description (max 60 chars)	Long Description (what it is, what it does, how to use)	Design Notes	Source (where does it come from?)
BBa_K195100	CD4 gene K195100	coding	1377	BBa_K195100	This part is human CD4 gene.	The coding sequence CD4 gene is a receptor on CD4 lymphocyte membrane, it may interact with HIV's gp120.		NYMU Dr. Tze-Tze Liu provided us the cDNA clone.
BBa_K195101	K195101 CD4 gene K195100 Terminator B0015	composite	1506	BBa_K195100 BBa_B0015	This part is composed of CD4 gene and terminator.	The coding sequence CD4 gene is a receptor on CD4 lymphocyte membrane, it may interact with HIV's gp120.
BBa_K195102	K195102 Lpp-OmpA-linker K103006 CD4 gene K195100 Terminator B0015	composite	1969	BBa_K103006 BBa_K195100 BBa_B0015	This part is composed of Lpp-OmpA-linker, CD4 gene and terminator.	Fusion CD4 and Lpp-ompA-Linker(BBa_K103006) together, so CD4 receptor can anchor on the outer membrane.
BBa_K195103	K195103 RBS B0034 Lpp-OmpA-linker K103006 CD4 gene K195100 Terminator B0015	composite	1981	BBa_B0034 BBa_K103006 BBa_K195100 BBa_B0015	This part is composed of RBS, Lpp-OmpA-linker, CD4 gene and terminator.	Fusion CD4 and Lpp-ompA-Linker(BBa_K103006) together, so CD4 receptor can anchor on the outer membrane.
BBa_K195104	K195104 pTet R0040 RBS B0034 Lpp-OmpA-linker K103006 CD4 gene K195100 Terminator B0015	composite	2035	BBa_R0040 BBa_B0034 BBa_K103006 BBa_K195100 BBa_B0015	This part is composed of pTet, RBS, Lpp-OmpA-linker, CD4 gene and terminator.	Fusion CD4 and Lpp-ompA-Linker(BBa_K103006) together, so CD4 receptor can anchor on the outer membrane.

Cellular Receptors: CCR5

Part Name	Picture	Part Type	Part Length	Subparts	Short Description (max 60 chars)	Long Description (what it is, what it does, how to use)	Design Notes	Source (where does it come from?)
BBa_K195105	CCR5 gene K195105	coding	1059	BBa_K195105	This part is human CCR5 gene.	The coding sequence CCR5 gene is a receptor on CD4 lymphocyte membrane, it may interact with HIV's gp120.		NYMU Dr. Tze-Tze Liu provided us the cDNA clone.
BBa_K195106	K195106 CCR5 gene K195105 Terminator B0015	composite	1188	BBa_K195105 BBa_B0015	This part is composed of CCR5 gene and terminator.	The coding sequence CCR5 gene is a receptor on CD4 lymphocyte membrane, it may interact with HIV's gp120.
BBa_K195107	K195107 Lpp-OmpA-linker K103006 CCR5 gene K195105 Terminator B0015	composite	1651	BBa_K103006 BBa_K195105 BBa_B0015	This part is composed of Lpp-OmpA-linker, CCR5 gene and terminator.	Fusion CCR5 and Lpp-ompA-Linker(BBa_K103006) together, so CD4 receptor can anchor on the outer membrane.
BBa_K195108	K195108 RBS B0034 Lpp-OmpA-linker K103006 CCR5 gene K195105 Terminator B0015	composite	1663	BBa_B0034 BBa_K103006 BBa_K195105 BBa_B0015	This part is composed of RBS, Lpp-OmpA-linker, CCR5 gene and terminator.	Fusion CCR5 and Lpp-ompA-Linker(BBa_K103006) together, so CD4 receptor can anchor on the outer membrane.
BBa_K195109	K195109 pTet R0040 RBS B0034 Lpp-OmpA-linker K103006 CCR5 gene K195105 Terminator B0015	composite	1717	BBa_R0040 BBa_B0034 BBa_K103006 BBa_K195105 BBa_B0015	This part is composed of pTet, RBS, Lpp-OmpA-linker, CCR5 gene and terminator.	Fusion CCR5 and Lpp-ompA-Linker(BBa_K103006) together, so CD4 receptor can anchor on the outer membrane.

K195200-K195299

Cellular Receptors: Integrin

Part Name	Picture	Part Type	Part Length	Subparts	Short Description (max 60 chars)	Long Description (what it is, what it does, how to use)	Design Notes	Source (where does it come from?)
BBa_K195200	alpha-V integrin gene K195200	coding	3039	BBa_K195200	This part is human alpha-V integrin gene.	The coding sequence alpha-V integrin is a receptor on human cell, it may interact with capsid protein penton base of several virus.		NYMU Dr. Tze-Tze Liu provided us the cDNA clone.
BBa_K195201	K195201 alpha-V integrin gene K195200 Terminator B0015	composite	3168	BBa_K195200 BBa_B0015	This part is composed of alpha-V integrin gene and terminator.	The coding sequence alpha-V integrin is a receptor on human cell, it may interact with capsid protein penton base of several virus.
BBa_K195202	K195202 Lpp-OmpA-linker K103006 alpha-V integrin gene K195200 Terminator B0015	composite	3631	BBa_K103006 BBa_K195200 BBa_B0015	This part is composed of Lpp-OmpA-linker, alpha-V integrin gene and terminator.	Fusion alpha-V integrin gene and Lpp-ompA-Linker(BBa_K103006) together, so alpha-V integrin receptor can anchor on the outer membrane.
BBa_K195203	K195203 RBS B0034 Lpp-OmpA-linker K103006 alpha-V integrin gene K195200 Terminator B0015	composite	3643	BBa_B0034 BBa_K103006 BBa_K195200 BBa_B0015	This part is composed of RBS, Lpp-OmpA-linker, alpha-V integrin gene and terminator.	Fusion alpha-V integrin gene and Lpp-ompA-Linker(BBa_K103006) together, so alpha-V integrin receptor can anchor on the outer membrane.
BBa_K195204	K195204 pTet R0040 RBS B0034 Lpp-OmpA-linker K103006 alpha-V integrin gene K195200 Terminator B0015	composite	3697	BBa_R0040 BBa_B0034 BBa_K103006 BBa_K195200 BBa_B0015	This part is composed of pTet, RBS, Lpp-OmpA-linker, alpha-V integrin gene and terminator.	Fusion alpha-V integrin gene and Lpp-ompA-Linker(BBa_K103006) together, so alpha-V integrin receptor can anchor on the outer membrane.

Cellular Receptors: CXADR(CAR)

Part Name	Picture	Part Type	Part Length	Subparts	Short Description (max 60 chars)	Long Description (what it is, what it does, how to use)	Design Notes	Source (where does it come from?)
BBa_K195205	CXADR gene K195205	coding	1098	BBa_K195206	This part is human CXADR gene.	The coding sequence CXADR receptor is a receptor on human cell, it may interact with capsid fiber protein of several virus.		NYMU Dr. Tze-Tze Liu provided us the cDNA clone.
BBa_K195206	K195206 CXADR gene K195205 Terminator B0015	composite	1227	BBa_K195200 BBa_B0015	This part is composed of CXADR gene and terminator.	The coding sequence CXADR receptor is a receptor on human cell, it may interact with capsid fiber protein of several virus.
BBa_K195207	K195207 Lpp-OmpA-linker K103006 CXADR gene K195205 Terminator B0015	composite	1690	BBa_K103006 BBa_K195200 BBa_B0015	This part is composed of Lpp-OmpA-linker, CXADR gene and terminator.	Fusion CXADR gene and Lpp-ompA-Linker(BBa_K103006) together, so CXADR receptor can anchor on the outer membrane.
BBa_K195208	K195208 RBS B0034 Lpp-OmpA-linker K103006 CXADR gene K195205 Terminator B0015	composite	1702	BBa_B0034 BBa_K103006 BBa_K195200 BBa_B0015	This part is composed of RBS, Lpp-OmpA-linker, CXADR gene and terminator.	Fusion CXADR gene and Lpp-ompA-Linker(BBa_K103006) together, so CXADR receptor can anchor on the outer membrane.
BBa_K195209	K195209 pTet R0040 RBS B0034 Lpp-OmpA-linker K103006 CXADR gene K195205 Terminator B0015	composite	1756	BBa_R0040 BBa_B0034 BBa_K103006 BBa_K195205 BBa_B0015	This part is composed of pTet, RBS, Lpp-OmpA-linker, CXADR gene and terminator.	Fusion CXADR gene and Lpp-ompA-Linker(BBa_K103006) together, so CXADR receptor can anchor on the outer membrane.

K195300-K195399

Cellular Receptors: Sialic Acid

Part Name	Picture	Part Type	Part Length	Subparts	Short Description (max 60 chars)	Long Description (what it is, what it does, how to use)	Design Notes	Source (where does it come from?)
BBa_K195300	neu gene operon K195300	coding	5229	BBa_K195300	This part is composed of neuD,neuB,neuA,neuC,neuE(neu gene cluster).	The coding sequence neu genes are operate in one operon in E.coli K1, which are coding for the enzymes that are involved in sialic acid synthesis.		NCKU provided us the E.coli K1.
BBa_K195301	K195301 neu gene operon K195300 Terminator B0015	composite		BBa_K195300 BBa_B0015	This part is composed of neu gene operon and terminator.
BBa_K195302	K195302 RBS B0034 neu gene operon K195300 Terminator B0015	generator		BBa_B0034 BBa_K195300 BBa_B0015	This part is composed of RBS,neu gene operon and terminator.	The coding sequence aim at sialic acid synthesis.
BBa_K195303	K195303 pTet R0040 RBS B0034 neu gene operon K195300 Terminator B0015	generator		BBa_R0040 BBa_B0034 BBa_K195300 BBa_B0015	This part is composed of pTet, RBS, neu gene operon and terminator.	The pTet promoter is strong enough to overexpress the neu gene operon.
BBa_K195304	sialyltransferase 0160 K195304	coding	2006	BBa_K195304	This part is the coding sequence of alpha-2,6-sialyltransferase.	alpha-2,6-sialyltransferase can transfer polysaccharides onto the cell membrane to fuse with the membrane proteins, which then form glycoproteins.
BBa_K195305	K195305 sialyltransferase 0160 K195304 Terminator B0015	composite		BBa_K195304 BBa_B0015	This part is composed of sialyltransferase 0160 and terminator.
BBa_K195306	K195306 RBS B0034 sialyltransferase 0160 K195304 Terminator B0015	generator		BBa_B0034 BBa_K195304 BBa_B0015	This part is composed of RBS,sialyltransferase 0160 and terminator.
BBa_K195307	K195307 pTet R0040 RBS B0034 sialyltransferase 0160 K195304 Terminator B0015	generator		BBa_R0040 BBa_B0034 BBa_K195304 BBa_B0015	This part is composed of pTet, RBS, sialyltransferase 0160 and terminator.	The pTet promoter is strong enough to overexpress the sialyltransferase 0160.

K195400-K195499

Cellular Receptors: Antibody

Part Name	Picture	Part Type	Part Length	Subparts	Short Description (max 60 chars)	Long Description (what it is, what it does, how to use)	Design Notes	Source (where does it come from?)
BBa_K195400	F10scFv K195400	Coding	840	K195400	single chain Fv (Antibody) that bind to Influenza	F10scFv can bind to the highly conserved region in HA on many subtypes of the Influenza A virus. F10scFv binds to a common epitope that is a highly conserved pocket in the stem region of Hemagglutinin containing the fusion peptide.		synthesis by Mr.gene
BBa_K195401	K195401 Lpp-OmpA-linker K103006 F10scFv K195400	composite	1304	BBa_K103006 BBa_K195400	Fusion BBa_K195400 with BBa_K103006	Fusion our BBa_K195400 with BBa_K103006, so the F10scFv can be anchored on surface of E. coli.	Scarless in-fusion by PCR
BBa_K195402	K195402 RBS B0034 Lpp-OmpA-linker K103006 F10scFv K195400	generator	1332	BBa_B0034 BBa_K103006 BBa_K195400	This part is composed of RBS,Lpp-OmpA-linker and F10scFv	The coding sequence can express F10scFv(BBa_K195400) on outer membrane by OmpA(BBa_K103006).
BBa_K195403	K195403 pTetR R0040 RBS B0034 Lpp-OmpA-linker K103006 F10scFv K195400	generator	1384	BBa_R0040 BBa_B0034 BBa_K103006 BBa_K195400	This part is composed of pTetR,RBS,Lpp-OmpA-linker and F10scFv	Fusion our BBa_K195400 with BBa_K103006, so our F10scFv can be transported to outer membrane of E. coli.
BBa_K195404	K195404 pTetR R0040 RBS B0034 Lpp-OmpA-linker K103006 F10scFv K195400 Terminator B0015	generator	1531	BBa_R0040 BBa_B0034 BBa_K103006 BBa_K195400 BBa_B0015	This part is composed of pTetR,RBS,Lpp-OmpA-linker,F10scFv and terminator	Fusion our BBa_K195400 with BBa_K103006, so our F10scFv can be transported to outer membrane of E. coli.
BBa_K195410	C-IgAP K195405	coding	1257	BBa_K195405	C-terminal of IgA protease	The leader sequence of C-IgAP is cleaved at the membrane by a signal peptidase, releasing the mature polyprotein into the periplasm. When in the periplasm, the beta-domain of the protein inserts into the outer membrane to form a beta-barrel structure. After formation of the beta-barrel pore, the passenger domain is translocated to the cell surface through the pore. Once at the cell surface, several possibilities may occur.		Neisseria gonorrhoeae FA1090(provide by Dr.Hsing-Ju Wu, Academia sinica/Institute of Biological Chemistry)
BBa_K195406	K195406 F10scFv K195400 C-IgAP K195406	composite	2097	BBa_K195400 BBa_K195406	This part is composed of F10scFv and C-IgAP	scFv fusion with C-IgAP
BBa_K195407	K195407 PelB J32015 F10scFv K195400	composite	906	BBa_J32015 BBa_K195400	This part is composed of PelB and F10scFv	scFv fusion with PelB
BBa_K195408	K195408 PelB J32015 F10scFv K195400 C-IgAP K195406	composite	2163	BBa_J32015 BBa_K195400 BBa_K195406	This part is composed of PelB,F10scFv and C-IgAP
BBa_K195409	K195409 RBS B0034 PelB J32015 F10scFv K195400 C-IgAP K195406	generator	2197	BBa_B0034 BBa_J32015 BBa_K195400 BBa_K195406	This part is composed of RBS,PelB,F10scFv and C-IgAP	The coding sequence express F10scFv on E.coli surface
BBa_K195412	K195410 RBS B0034 PelB J32015 F10scFv K195400 C-IgAP K195406 Terminator B0015	generator	2334	BBa_B0034 BBa_J32015 BBa_K195400 BBa_K195406 BBa_B0015	This part is composed of RBS,PelB,F10scFv,C-IgAP and terminator	The coding sequence express F10scFv on E.coli surface
BBa_K195411	K195411 pTetR R0040 RBS B0034 PelB J32015 F10scFv K195400 C-IgAP K195406 Terminator B0015	generator	2396	BBa_B0034 BBa_J32015 BBa_K195400 BBa_K195406 BBa_B0015	This part is composed of pTetR,RBS,PelB,F10scFv,C-IgAP and terminator	The coding sequence express F10scFv on E.coli surface

K195600-K195699

Removal

Part Name	Picture	Part Type	Part Length	Subparts	Short Description (max 60 chars)	Long Description (what it is, what it does, how to use)	Design Notes	Source (where does it come from?)
BBa_K195600	K195600 CII K116602 Terminator B0015	composite	431	BBa_K116602 BBa_B0015	This part is composed of CII and terminator.	The coding sequence CII could activate BBa_K116603,the regulatory promoter pRE from λ phage.		This part comes from IGEM_08 NYMU-Taipei.
BBa_K195601	K195601 RBS B0034 CII K116602 Terminator B0015	generator	449	BBa_B0034 BBa_K116602 BBa_B0015	This part is composed of RBS,CII and terminator.	The coding sequence CII could activate BBa_K116603,the regulatory promoter pRE from λ phage.
BBa_K195602	K195602 TetR C0040 Terminator B0015	composite	822	BBa_C0040 BBa_B0015	This part is composed of TetR and terminator.	The coding sequence TetR could repress the regulatory promoter pTetR(BBa_R0040).
BBa_K195603	K195603 RBS B0034 TetR C0040 Terminator B0015	generator	840	BBa_B0034 BBa_C0040 BBa_B0015	This part is composed of RBS,TetR and terminator.	The coding sequence TetR could repress the regulatory promoter pTet(BBa_R0040).
BBa_K195604	K195604 PenI C0074 Terminator B0015	composite	585	BBa_C0074 BBa_B0015	This part is composed of PenI and terminator.	The coding sequence PenI could repress regulatory promoter pPenI(BBa_R0074).
BBa_K195605	K195605 RBS B0034 PenI C0074 Terminator B0015	generator	603	BBa_B0034 BBa_C0074 BBa_B0015	This part is composed of RBS,PenI and terminator.	The coding sequence PenI could repress regulatory promoter pPenI(BBa_R0074).
BBa_K195606	K195606 pTetR R0040 RBS B0034 CII K116602 Terminator B0015	generator	511	BBa_R0040 BBa_B0034 BBa_K116602 BBa_B0015	This part is composed of pTetR, RBS,CII and terminator.	The coding sequence CII could activate BBa_K116603,the regulatory promoter pRE from λ phage. The TetR repressible promoter is repressed by TetR (BBa_C0040).
BBa_K195607	K195607 pPenI R0074 RBS B0034 CII K116602 Terminator B0015	generator	534	BBa_R0074 BBa_B0034 BBa_K116602 BBa_B0015	This part is composed of pPenI, RBS,CII and terminator.	The coding sequence CII could activate BBa_K116603,the regulatory promoter pRE from λ phage. The PenI repressible promoter is repressed by PenI (BBa_C0074).
BBa_K195608	K195608 pLacI R0010 RBS B0034 CII K116602 Terminator B0015	generator	657	BBa_R0040 BBa_B0034 BBa_K116602 BBa_B0015	This part is composed of pLacI, RBS,CII and terminator.	The coding sequence CII could activate BBa_K116603,the regulatory promoter pRE from λ phage.
BBa_K195609	K195609 pRE K116603 RBS B0034 GFP E0040 Terminator B0015	Reporter	931	BBa_K116603 BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015	This part is composed of pRE and GFP generator.	The promoter pRE could be activated by BBa_K116602,the activator CII.
BBa_K195610	K195610 pTet R0040 RBS B0034 GFP E0040 Terminator B0015	Reporter	937	BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015	This part is composed of pTet and GFP generator.	The promoter pTet could be repressed by BBa_C0040,the repressor TetR.
BBa_K195611	K195611 pPenI R0074 RBS B0034 GFP E0040 Terminator B0015	Reporter	960	BBa_R0074 BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015	This part is composed of pPenI and GFP generator.	The promoter pPenI could be repressed by BBa_C0074,the repressor PenI.
BBa_K195612	K195612 pLacI R0010 RBS B0034 GFP E0040 Terminator B0015	Reporter	1083	BBa_R0010 BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015	This part is composed of pLacI and GFP generator.	The pLacI is repressed by LacI, which could be inhibited by IPTG.
BBa_K195613	K195613 pCI R1051 RBS B0034 GFP E0040 Terminator B0015	Reporter	932	BBa_R1051 BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015	This part is composed of pCI and GFP generator.	The promoter pCI could be repressed by BBa_C0051,the repressor CI from E.coli phage lambda.
BBa_K195614	K195614 p22 R0053 RBS B0034 GFP E0040 Terminator B0015	Reporter	937	BBa_R0053 BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015	This part is composed of p22 and GFP generator.	The promoter p22 could be repressed by BBa_C0053,the c2 repressor from Salmonella phage P22 .
BBa_K195615	K195615 pLuxR R0074 RBS B0034 GFP E0040 Terminator B0015	Reporter	938	BBa_R0074 BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015	This part is composed of pLuxR and GFP generator.	The promoter pLuxR could be activated by LuxR in concert with HSL.
BBa_K195616	K195616 pLasR R0079 RBS B0034 GFP E0040 Terminator B0015	Reporter	1040	BBa_R0074 BBa_R0040 BBa_B0034 BBa_E0040 BBa_B0015	This part is composed of pLasR and GFP generator.	The promoter pLasR could be repressed by BBa_C0079,the repressor LasR.
BBa_K195617	K195617 pTetR R0040 RBS B0034 CII K116602 Terminator B0015 pRE K116603 RBS B0034 GFP E0040 Terminator B0015	composite	1450	BBa_R0040 BBa_B0034 BBa_K116602 BBa_B0015 BBa_K116603 BBa_B0034 BBa_E0040 BBa_B0015	This part is composed of pTetR with activator gene CII, and it would activate pRE to produce green fluorescent protein.	The coding sequence CII could activate BBa_K116603,the regulatory promoter pRE from λ phage. The TetR repressible promoter is repressed by TetR (BBa_C0040).The pRE would be activated by CII produced by the downstream gene of the regulatory promoter pTet and produce green fluorescent protein.
BBa_K195618	K195618 pPenI R0074 RBS B0034 CII K116602 Terminator B0015 pRE K116603 RBS B0034 GFP E0040 Terminator B0015	composite	1473	BBa_R0074 BBa_B0034 BBa_K116602 BBa_B0015 BBa_K116603 BBa_B0034 BBa_E0040 BBa_B0015	This part is composed of pPenI with activator gene CII, and it would activate pRE to produce green fluorescent protein.	The coding sequence CII could activate BBa_K116603,the regulatory promoter pRE from λ phage. The PenI repressible promoter is repressed by PenI (BBa_C0074).The pRE would be activated by CII produced by the downstream gene of the regulatory promoter pPenI and produce green fluorescent protein.
BBa_K195619	K195619 pLacI R0010 RBS B0034 CII K116602 Terminator B0015 pRE K116603 RBS B0034 GFP E0040 Terminator B0015	composite	1596	BBa_R0010 BBa_B0034 BBa_K116602 BBa_B0015 BBa_K116603 BBa_B0034 BBa_E0040 BBa_B0015	This part is composed of pLacI with activator gene CII, and it would activate pRE to produce green fluorescent protein.	The coding sequence CII could activate BBa_K116603,the regulatory promoter pRE from λ phage. The pLacI is repressed by LacI, which could be inhibited by IPTG.The pRE would be activated by CII produced by the downstream gene of the regulatory promoter pLacI and produce green fluorescent protein.
BBa_K195620	K195620 pRE K116603 RBS B0034 TetR C0040 Terminator B0015	generator	896	BBa_K116603 BBa_B0034 BBa_C0040 BBa_B0015	This part is composed of pRE and TetR repressor.	The promoter pRE could be activated by BBa_K116602,the activator CII. TetR could repress the regulatory promoter BBa_R0040 pTet.
BBa_K195621	K195621 pRE K116603 RBS B0034 PenI C0074 Terminator B0015	generator	659	BBa_K116603 BBa_B0034 BBa_C0074 BBa_B0015	This part is composed of pRE and PenI repressor.	The promoter pRE could be activated by BBa_K116602,the activator CII. PenI from Bacillus licheniformis with LVA tail could repress the regulatory promoter BBa_R0074 pPenI.
BBa_K195622	K195622 pRE K116603 RBS B0034 TetR C0040 Terminator B0015 pTetR R0040 RBS B0034 CII K116602 Terminator B0015 pRE K116603 RBS B0034 GFP E0040 Terminator B0015	Device	2354	BBa_K116603 BBa_B0034 BBa_C0040 BBa_B0015 BBa_R0040 BBa_B0034 BBa_K116602 BBa_B0015 BBa_K116603 BBa_B0034 BBa_E0040 BBa_B0015	This part is a damped oscillator by using the regulatory promoter and the interaction between the activator, repressor and regulatory promoter.	This device is produces damped oscillations between TetR and CII. The pRE (K116603) can be activated by CII (K116602), which in turn produces TetR (C0040) and GFP. The TetR then represses the pTet promoter (R0040) resulting in slower production of CII. This design was based on the design of a relaxation oscillator (two component oscillator). This design does not produce sustained oscillations like a normal oscillator (relaxation based or otherwise) because of the two oscillating effectors not being able to directly affect each other.
BBa_K195623	K195623 pRE K116603 RBS B0034 PenI C0074 Terminator B0015 pPenI R0074 RBS B0034 CII K116602 Terminator B0015 pRE K116603 RBS B0034 GFP E0040 Terminator B0015	Device	2140	BBa_K116603 BBa_B0034 BBa_C0074 BBa_B0015 BBa_R0074 BBa_B0034 BBa_K116602 BBa_B0015 BBa_K116603 BBa_B0034 BBa_E0040 BBa_B0015	This part is a time delay device using the regulatory promoter and the interaction between the activator, repressor and regulatory promoter.	This device is produces damped oscillations between PenI and CII. The pRE (K116603) can be activated by CII (K116602), which in turn produces PenI (C0074) and GFP. The PenI then represses the pPenI promoter (R0074) resulting in slower production of CII. This design was based on the design of a relaxation oscillator (two component oscillator). This design does not produce sustained oscillations like a normal oscillator (relaxation based or otherwise) because of the two oscillating effectors not being able to directly affect each other.
BBa_K195624	K195624 pRE K116603 RBS B0034 TetR C0040 Terminator B0015 pTetR R0040 RBS B0034 CII K116602 Terminator B0015	Device	1415	BBa_K116603 BBa_B0034 BBa_C0040 BBa_B0015 BBa_R0040 BBa_B0034 BBa_K116602 BBa_B0015	This main part of the time delay device.	The promoter pRE could be activated by BBa_K116602,the activator CII, which results in the production of the TetR and green fluorescent protein. TetR could repress the regulatory promoter BBa_R0040 pTet, which results in the lower production of the activator CII.
BBa_K195625	K195625 pRE K116603 RBS B0034 PenI C0074 Terminator B0015 pPenI R0074 RBS B0034 CII K116602 Terminator B0015	Device	1210	BBa_K116603 BBa_B0034 BBa_C0074 BBa_B0015 BBa_R0074 BBa_B0034 BBa_K116602 BBa_B0015	This main part of the time delay device.	The promoter pRE could be activated by BBa_K116602,the activator CII, which results in the production of the TetR and green fluorescent protein. PenI from Bacillus licheniformis with LVA tail could repress the regulatory promoter BBa_R0074 pPenI, which results in the lower production of the activator CII.
BBa_K195626	K195626 pTetR R0040 RBS B0034 PenI C0074 Terminator B0015	generator	665	BBa_R0040 BBa_B0034 BBa_C0074 BBa_B0015	This part is composed of pTetR, RBS,PenI and terminator.	The coding sequence PenI could repress BBa_R0074,the regulatory promoter pPenI. This is a subunit of toggle switch. pTet could be repressed by repressor TetR (BBa_C0040).
BBa_K195627	K195627 pPenI R0074 RBS B0034 TetR C0040 Terminator B0015	generator	925	BBa_R0074 BBa_B0034 BBa_C0040 BBa_B0015	This part is composed of pPenI, RBS,TetR and terminator.	The coding sequence TetR could repress BBa_R0040,the regulatory promoter pTet. This is a subunit of toggle switch. pPenI could be repressed by repressor PenI (BBa_C0074).
BBa_K195628	K195628 pTetR R0040 RBS B0034 PenI C0074 Terminator B0015 pPenI R0074 RBS B0034 TetR C0040 Terminator B0015	Device	1207	BBa_R0040 BBa_B0034 BBa_C0074 BBa_B0015 BBa_R0074 BBa_B0034 BBa_K116602 BBa_B0015	This part is a toggle switch.	The coding sequence PenI could repress BBa_R0074,the regulatory promoter pPenI.The coding sequence TetR could repress BBa_R0040,the regulatory promoter pTet.
BBa_K195629	K195629 Cl lam C0051 Terminator B0015	composite	912	BBa_C0051 BBa_B0015	This part is composed of cl repressor and terminator.	The coding sequence cl repressor from bacteriophage lambda with LVA tail could repress BBa_R1051,the regulatory promoter pCI.
BBa_K195630	K195630 RBS B0034 Cl C0051 Terminator B0015	generator	930	BBa_B0034 BBa_C0051 BBa_B0015	This part is composed of RBS,cl repressor and terminator.	The coding sequence cl repressor from bacteriophage lambda with LVA tail could repress BBa_R1051,the regulatory promoter pCI.

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