• Home
• Team
• Project
• Parts
• Modelling
• Sponsors
• Lab Book

Project

• Overview
• Choices Rationale
• Characterisation
• Safety
• Judging Comments

Sub-projects

• Metal Intake/Efflux
• Metal Sensing
• Sporulation Tuning
• Population Dynamics
• Stochastic Switch
• Metal Sequester
• Chassis
• Promoter Library

Wet Lab

• Lab Book
• Protocols

Meetings

• Meetings Calendar

Planning

• Timeline
• Ideas

Links

• Helping other teams
• Ethics
• T-Shirts
• iGEM Meetup
• Our City
• Fun and Games
• Sponsors
• Useful Links
• Contact Us
• Press Coverage

Judging Comments

This year, despite our relatively ambitious project, our team achieved goals in several different areas.

In addition to successfully modelling, designing, characterising and entering our sporulation tuning kinA BioBrick (BBa_K174011) in the parts registry, we improved an existing BioBrick part (BBa_K174004) rendering it tightly regulated.

Futhermore, we helped the UQ-Australia iGEM2009 team by modelling their system.

We also modelled all the components of our project's system, and we designed and entered 16 other BioBrick parts and devices, ranking from a degradation controller system to a tightly regulated metal sensing promoter. We believe that several of the BioBrick parts we designed, in addition to our three favourite parts, are novel and can be used not only in the context of our project but in a variety of different systems.

Our degradation controller device is arabinose inducible and can be used more widely to tightly regulate the levels of protein in a particular system. In addition to transcriptional and translational regulation widely used in the Synthetic Biology area, our device allows us to have a third control over the level of a protein of interest.

In addition, our sensitive cadmium promoter BioBrick is novel in the fact that there is no known cadmium specific promoter. Our combinatorial approach to use two different transcriptional repressor proteins in order to design our cadmium specific promoter is both unique and novel and provide us with a tightly regulated promoter which any system working with cadmium can use.