Team:BIOTEC Dresden/Results Vesicles

Vesicle formation in Microfluidic Chamber

The video shows the formation of vesicles in a V-chamber. The surfactant Span 80 at a concentration (V/V) of 1% was dissolved in the oil phase. The flow rates used were different for every chamber used and are hence not transferable to other systems. Vesicle size varied with applied flow rates: increased flow rates of the aqueous phase yielded in larger vesicles. Above a critical flow rate the aqueous phase would displace the oil phase.

Vesicle formation in V-chamber. Surfactant: 1% Span 80

The vesicles have been observed in the monitoring section of the microfluidic chamber. Figure 1 shows a typical vesicle moving through a channel.

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The typical diameter of the vesicles was 15 µm, the volume under the reasonable assumtion of spherical shape was around 12,5 pl.

A microfluidic chamber that created an intersection between aqueous and oil phases produced vesicles that were uniform in size and stable for up to 5 hours. The vesicles are stabilized by a surfactant, Span 80.

Vesicles are led into a grid by funnel shaped structures in the flow chamber (top).

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