13th July

Weenink

prepared 10 agar+amp plates

Also did the first transformations to see how it goes:

BBa_K145280 (GFP-LVA + TetR generators)
BBa_K145279 (GFP + TetR generators)

4 vials of DH5alpha comptetent cells were used (2 for each biobrick)

after addition of 1 µl rehydrated biobrick DNA, the tubes were incubated for approx 15 (±5) min. on ice. Then one tube of each transformation was heatshocked for 1 min at 42 degrees.

All four transformations were plated out and put at 37 degrees (at 19:00)
BBa_K145280 no heatshock approx 80 microliter on agar+amp plate
BBa_K145279 no heatshock 50 microliter on agar+amp plate
BBa_K145280 heatshocked 50 microliter on agar+amp plate
BBa_K145279 heatshocked 50 microliter on agar+amp plate