17th July

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9:45 inoculated 30 ml LB amp in 37ºC shaking incubator
pSB1AK3
pSB1AC3
pSB1AT3

11:30 purified plasmids
(in conctrary to protocol i accidentally added >350µl lysis buffer, so i also added 450µl neutralisation buffer) Yield of 100 µl elution:
BBa_B0032: 21.2 ng/µl
BBa_K145015: 33.0 ng/µl
BBa_K145201: 27.8 ng/µl

BBa_K145280 30ml culture induced with 1mM IPTG at 14:40