17th July

Weenink

9:45 inoculated 30 ml LB amp in 37ºC shaking incubator
pSB1AK3
pSB1AC3
pSB1AT3

11:30 purified plasmids
(in conctrary to protocol i accidentally added >350µl lysis buffer, so i also added 450µl neutralisation buffer) Yield of 100 µl elution:
BBa_B0032: 21.2 ng/µl
BBa_K145015: 33.0 ng/µl
BBa_K145201: 27.8 ng/µl

BBa_K145280 30ml culture induced with 1mM IPTG at 14:40

Calin

Riboregulator scheme 1 and scheme 2 Matlab scripts finished. Ordered lambda red plasmids, (pKD46, pKD3, pKD4) from [http://cgsc2.biology.yale.edu Yale CGSC]. Helped Sriram with transformations. Used DH5alpha comptetent cells to transform:
BBa_I714031 - OriT-R
BBa_J23100 - strong promoter
BBa_E0840 - GFP generator
BBa_I13522 - pTet GFP