Team:UNIPV-Pavia/Notebook/Week4Jul

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December 2008
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March 2009
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30 31
April 2009
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May 2009
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June 2009
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July 2009
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August 2009
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September 2009
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October 2009
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November 2009
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Week from July 20th, to July 26th, 2009

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July, 20th

  • Overnight digestion (20 ul reaction volume) for:
MRGENE1(X-P) MRGENE1-2(X-P) MRGENE1-3(X-P)
MRGENE2(X-P)(X2) MRGENE2-2(X-P) MRGENE2-3(X-P)
B0030(S-P)(X3)
  • NOTE: reactions which involve excision(X-P) were performed on 3 ug of DNA, while S-P digestions were performed with 1 ug of DNA. We decided to have 3 replicates to be sure to have an acceptable yield.
  • We decided to perform gel run/cut/purification for MRGENE2 and for B0030, while we decided to perform DNA precipitation with sodium acetate for MRGENE1.


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July, 21st

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July, 22nd

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July, 23rd

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July, 24th

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