Team:TUDelft/23 July 2009

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Revision as of 19:04, 23 July 2009 by Tim Weenink (Talk | contribs)

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Tim Weenink

Details of the following to be provided tomorrow:

grew up *S in LC

looked at gel

Did !A construction with double the amount of backbone (and rest from fridge). Did it with both DH5alpha and homemade top10 chemically competent cells.

PCR'd 8 colonies from my transformation plates with VF2 and I-SceI Reverse primers to see if there was incorporation of the I-SceI restriction site

isolated plasmid from *S

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