Team:TUDelft/23 July 2009

From 2009.igem.org

Revision as of 19:09, 23 July 2009 by Tim Weenink (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Tim Weenink

Details of the following to be provided tomorrow:

grew up *S in LC

looked at gel

Did !A assembly again with double the amount of backbone (and rest from fridge). Did it with both DH5alpha and homemade top10 chemically competent cells, to compare transformation efficiencies. Also the right concentration of K was used

PCR'd 8 colonies from my transformation plates with VF2 and I-SceI Reverse primers to see if there was incorporation of the I-SceI restriction site

isolated plasmid from *S

Retrieved from "http://2009.igem.org/Team:TUDelft/23_July_2009"