Team:Groningen/Promoters

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[http://2009.igem.org/Team:Groningen http://2009.igem.org/wiki/images/f/f1/Igemhomelogo.png]

This page shows the measurements we did of our constitutive promoters and derives approximate RPUs relative to the weakest promoter we use. For briefness the lines in the graphs use the original numbering, which can be summarized as follows:

Plasmid/Part → [http://partsregistry.org/Part:pSB1AC3 pSB1AC3] [http://partsregistry.org/Part:pSB3K3 pSB3K3] [http://partsregistry.org/Part:BBa_J23109 BBa_J23109] [http://partsregistry.org/Part:BBa_J23106 BBa_J23106] [http://partsregistry.org/Part:BBa_J23100 BBa_J23100] control
Medium → LB TY LB TY LB TY LB TY LB TY LB TY
Dilution (of culture) ↓ 1 7 2 8 3 9 4 10 5 11 6 12
100x A
B
200x C
D
400x E
F

Background

The following graphs show the optical density and fluorescence measurements for the controls. As can be seen 4 of the LB medium controls were probably contaminated, so these are ignored in the rest of the computations.

LB controls TY controls
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Based on the non-contaminated control measurements we find the following average background values:

  • LB
    • OD600: 0.094262500378219 (A, F)
    • Fluorescence: -11.4090909090909 (A)
    • Fluorescence: -20.6136363636364 (B,C,D,E,F)
  • TY
    • OD600: 0.09115113622763 (A-F)
    • Fluorescence: -9.06818181818182 (A)
    • Fluorescence: -20.05 (B,C,D,E,F)

Using the above background values we get the graphs below.

BBa_J23109 BBa_J23106 BBa_J23100
LB
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TY
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LB
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TY
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