Team:Newcastle/Labwork/27 July 2009
From 2009.igem.org
Today we prepared the gel for last week's rescued plasmids whcih contain RFP and GFP biobricks. WE prepared the gel with 0.8% agorose. The final solution was 500ml using 4 gr of agorose in total.
We checked the agar plates containing colonies of plasmids with five different biobricks.
Plate | BioBrick |
Plate 1 | Bba_C0056 |
Plate 2 | Bba_B1002 |
Plate 3 | Bba_?? |
Plate 4 | Bba_C0076 |
Plate 5 | Bba_R0077 |
Expcept from plate 3 we had colonies formed in all plates.
we prepared 12 tubes for 4 different biobricks. Colonies were picked and mixed in a tube containing 5ml of LB. Finally we left them at shaking incubator overnight. We labelled the tubes as below: 1A: 1B: 1C: We then prepared the plates for the plasmids.
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