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Team:EPF-Lausanne/Notebook/Wet Lab
From 2009.igem.org
Wet Lab
27.07.09
LOVTAP gene have been amplified again and runned on a gel to be sure to have the correct insert.
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015 (terminator)] was digested, runned on a gel (which displayed the correct band) and extracted with the gel extraction kit, this step didn't worked well.
LOVTAP gene have been than digested, purified and runned on a gel.
We already had a digested [http://partsregistry.org/Part:BBa_B0015 BBa_B0015 (terminator)].
Finally we ligated overnight at 4°C the digested LOVTAP in both [http://partsregistry.org/Part:BBa_B0015 terminators], the one digested-extracted today and the one already digested-extracted.
