Uppsala-Sweden/7 August 2009

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Overview sheet

Ligation of ADH2(Z),pirB, pdc, PpetE, adh2(Y) into pSB1A3

Ligation with the pSB1A3 vector using the Quick Ligation Kit from [http://www.neb.com/nebecomm/products/protocol2.asp NEB]. Calculated amounts can be found here Media:Ligation Calc 07082009.xls

Transformation of ADH2(Z),pirB, pdc, PpetE, adh2(Y) into competent E. coli cells

Transformation was performed as written in the protocol form the Ligation kit from [http://www.neb.com/nebecomm/products/protocol3.asp NEB]

--Karl.brune 08:56, 10 August 2009 (UTC)