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Team:Newcastle/Labwork/11 August 2009
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Lab Session 11/08/09
Chassis team
- Recreated LB inoculated plates
==Stochastic Switch Team Today we run the gel for our gfp-rrnb integration vector. We had restriction digest using EcoRI and HindIII. This gave us a 500bp of DNA on the gel.
Preparation of the gel
- 3.2 gr of agarise were solved in 400ml of 1xTAE buffer to make 0.8% agarose gel
- The solution was microwaved for 5 minutes and left to cool down
Restriction digest
- We prepared a final solution of 10ul for the restriction digest
- We used
- 4.75ul H2O
- 1ul 10xBuffer(BufferE)
- 0.25ul BSA
- 0.5ul HindIII
- 0.5ul EcoRI
- 3ul DNA (gfp-rrnb)
Running the gel
Results
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
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- Newcastle iGEM Twitter
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