Team:Newcastle/Labwork/12 August 2009
From 2009.igem.org
Lab Session 11/08/09
Stochastic Switch Team
Today we are trying to transform Bacillus subtilis with gfp-rrnb integration vector. Metal sensor team kindly inoculated B. subtilis cells into flask tubes and placed them into the shaking incubator. We labelled them as 1,2,3 and control.
We prepared four plates
- LB + Bsubtilis (+ Control, cells should grow on this plate)
- LB + CHL + B. subtilis (- control, cells will not grow on this plate)
- LB + CHL + B. subtilis + plasmid DNA(Cells should transform with the DNA)
- LB + CHL + B. subtilis + diluted plasmid DNA (Cells should transform with the DNA)
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
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