Team:Newcastle/Labwork/12 August 2009

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Lab Session 11/08/09

Stochastic Switch Team

Today we are trying to transform Bacillus subtilis with gfp-rrnb integration vector. Metal sensor team kindly inoculated B. subtilis cells into flask tubes and placed them into the shaking incubator. We labelled them as 1,2,3 and control.

We prepared four plates

  • LB + Bsubtilis (+ Control, cells should grow on this plate)
  • LB + CHL + B. subtilis (- control, cells will not grow on this plate)
  • LB + CHL + B. subtilis + plasmid DNA(Cells should transform with the DNA)
  • LB + CHL + B. subtilis + diluted plasmid DNA (Cells should transform with the DNA)



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