Team:Newcastle/Project/Labwork/OurProtocols/Making Agar Plates

From 2009.igem.org

Revision as of 12:32, 14 August 2009 by Goksel (Talk | contribs)

Preparing the LB+agar powder

Mix the contents below to prepare the agar powder required to make 10L of agar solution

  • 100gr tryptoptan
  • 50gr yeast extract
  • 50gr NaCl
  • 150 gr agar

Mix the content very well before using the mixed powder

Preparing the LB+agar solution

To prepare 1L of LB+agar solution

  • Put 500 ml of distilled water to a tube
  • Put 400ml of distilled water to a tube and 35 gr of LB+agar powder. Top the tube up to 500ml with distilled water
  • Add 10ul of antifoam to LB+agar tube
  • Put the tubes for autoclaving for half an hour
  • Put the tube with LB+agar into 55C-60C water bath.
  • Put the water tube into the sink to cool it down.
  • If necessary, add the antibiotics to LB+agar tube after the tube cools down.
  • Mix both tubes throughly

Pouring the plates

  • Pour the plates using roughly 25ml of the solution for each Petri dish using the aseptic technique
  • Leave the dishes for 30 minutes in room temperature
  • Put them into 42C incubator with their lids off to dry them off
  • If you are not using the dishes straight away leave them on the bench for a day before placing them to the fridge





News

Events

Social Net

  • Newcastle iGEM Twitter
  • [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
  • [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]
Retrieved from "http://2009.igem.org/Team:Newcastle/Project/Labwork/OurProtocols/Making_Agar_Plates"