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Week 7

Monday

Wet Work

Amplification

Colony PCR of 8 colonies picked from 391 in pSB3K3 transformants, 8 colonies picked from 374 in pSB3K3 transformants, and 1 colony picked from 381 in pSB3K3 transformants, overnight cultures of each of those colonies as well.

MelA BioBrick

PCR done of the origonal MelA and the MelA inbtermediate to biobrick (without the second PstI site). This was too create enough DNA for a resitriction digest to be carried out to check that the previous weeks PCRs had been sucessful. After PCR, the products were run on a gel and extracted.

We also tested the Primer C again, as it didn't work last time. Three PCR's were done:

• Primer F and Primer C used last time
• Primer F and a new aliquot of Primer C
• Primer F and revortexed and re-aliquoted primer C from the origonal vial

Dry Work

We found the phzM and phzS pigments in the registry!

• BBa_I723024 (phzM)
• BBa_I723025 (phzS)

This means we can now start working on these pigments, connecting them to processing and logic systems.