Template:Team:KULeuven/24 August 2009/BlueLightReceptor

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  1. platen met ligatie A zijn onder het blauw licht gezet. De LEDs werden op maximum gezet.
  2. restriction digest with
    • tubes (1,3,5,7,9) of ligA (BLP + E0240) cut with EcoRI en PstI
    • promotor J23101 cut with EcoRI en SpeI (4x)
  3. gel electroforese with the RD of ligA and J23101 followed by a gel extraction:
    • note: tube 5 of ligA was loaded poorly on the gel and could not be used.
    • note: the samples of promotor J23101 were barly visible. only 2 of the 4 samples were recoverd by extraction.
Part concentration (ng/μl) 260/280 λ
ligA 1 7,9 2,16
ligA 3 10,8 1,88
ligA 7 4,8 2,49
ligA 9 8,0 3,02
(A) 20,8 1,70
(B) 12,8 2,57

3.enting of liquid cultures with kanamycin and pSB3K3

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