From 2009.igem.org
- platen met ligatie A zijn onder het blauw licht gezet. De LEDs werden op maximum gezet.
- restriction digest with
- tubes (1,3,5,7,9) of ligA (BLP + E0240) cut with EcoRI en PstI
- promotor J23101 cut with EcoRI en SpeI (4x)
- gel electroforese with the RD of ligA and J23101 followed by a gel extraction:
- note: tube 5 of ligA was loaded poorly on the gel and could not be used.
- note: the samples of promotor J23101 were barly visible. only 2 of the 4 samples were recoverd by extraction.
| Part | concentration (ng/μl) | 260/280 λ |
|
| ligA 1 | 7,9 | 2,16 |
|
| ligA 3 | 10,8 | 1,88 |
|
| ligA 7 | 4,8 | 2,49 |
|
| ligA 9 | 8,0 | 3,02 |
|
| (A) | 20,8 | 1,70 |
|
| (B) | 12,8 | 2,57 |
|
4.enting of liquid cultures with kanamycin and pSB3K3
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