Template:Team:KULeuven/27 August 2009/BlueLightReceptor

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Revision as of 13:02, 27 August 2009 by AnneV (Talk | contribs)
  1. the electroporations of 26/08 were checked.
    • pSB3K3 had some colonies. they are ented in liquid culture today.
    • LigC ( + ) did not grow. we figured that an insert of 35bp was to short to ligate so we decided to use as insert in stead of vector. the following restriction digest was started:
      • was cut with SpeI and PstI
      • was cut with XbaI and PstI
  2. the miniprep that was made to sequence the LigA construct on 26/08 was used again to perform a restriction digest (EcoRI and PstI). this was put on gel to check wether there actually was LigA-insert in the vector and wether the insert had the right lenght
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