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Overview Sensitivity Tuner --- Characterisation --- Modelling Colour Generators --- Carotenoids (Orange/Red) --- Melanin (Brown) --- Violacein (Purple/Green) The Future Safety

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Carotenoids

Our Action Plan

In our project this year, we aim to incorporate the carotenoid biosynthesis pathway in E. coli and produce different coloured pigments. Potential candidates are lycopene (red), beta-carotene (orange), and zeaxanthin (yellow). Since these three compounds are sequential in the biosynthetic pathway, we hope to demonstrate colour changes from red to orange to yellow, by controlling expression of the enzymes involved in conversion.

Our action plan is as follows:

1. Test the activity of K152005 (CrtEBIY).

2. Test pigment production in E. coli strain MG1655 and its derivative (up-regulation in precursors).

3. Construct composite biobrick containing CrtE, CrtB and CrtI (i.e. CrtEBI) and test its activity.

4. Transform E. coli with CrtEBI and CrtY. Test if activation of CrtY can cause colour changes in vivo.

5. Characterise our composite biobricks and the colour output.

6. Test compatibility with biobricks from previous iGEM teams and see if we can convert their outputs into pigment production.

Our Action Plan (Updated regularly). Green: completed. Yellow: Work in progress. Blue: Partially completed. Red: Planning.