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EPF-Lausanne/29 August 2009
From 2009.igem.org
Contents |
Wet Lab
Glycerol stock of the following clones :
-BB5-RO2#5, clone #1 ("double" construction)
-BB5-RO2#5, clone #4 ("double" construction)
-RO2#4 + BB1, clone #3 (double transformation)
-RO2#10 + BB6, clone #8 (double transformation)
They were put in the -80°C freezer (2nd box).
Although so far the colony PCR on these RO + BB didn't show anything concluding, the fact that they grew in antibiotic selection seems to show that it actually worked.
We also re-plated the double transformation clones on newly made plates containing both AMP and KANA. It grew, so it seems to indicate that what we have is right.
... to be continued !!
People in the lab
Basile (Saturday !)
